@article {7,
	title = {Morphology and molecular characterization of a new species of thecate benthic dinoflagellate, Coolia malayensis sp. nov. (Dinophyceae)},
	journal = {Journal of Phycology},
	volume = {46},
	year = {2010},
	month = {02/2010},
	pages = {162-171},
	chapter = {162},
	abstract = {Coolia Meunier is an important component of benthic dinoflagellate assemblages in tropical and subtropical seas. In this study, detailed morphological observation of Coolia species from Malaysian waters was carried out using light and electron microscopy in parallel with molecular characterization of nuclear-encoded partial LSU rDNA, and internal transcribed spacer (ITS) regions. Live specimens were collected from seaweed samples and established into clonal cultures. There are significant morphologial variations between the Malaysian isolates in comparison to the type species, C. monotis Meunier. the feature that differentiates the new species is the third postcingular plate (3{\textquoteright}{\textquoteright}{\textquoteright}), which is the largest hypothecal plate in the Malaysian isolates, whereas in C. monotis, the 3{\textquoteright}{\textquoteright}{\textquoteright} and 4{\textquoteright}{\textquoteright}{\textquoteright} plates are almost equal in size. Detailed observations of the thecal pores also revealed the presence of fine perforations within the pores of the Malaysian isolates, but these perforations are absent in C. monotis. Comparisons between Malaysian isolates and C. monotis nucleotide sequence of the ITS region showed high genetic divergence at 28\%, in contrast to the 0.3\%-3\% divergence observed among populations of the same species. Structural comparison of the second internal transcribed spacer (ITS2) rRNA transcript between the two species showed compensatory base changes (CBCs) in the three helices of ITS2 rRNA. Based on morphological and molecular data, the Malaysian isolates are considered to represent a new species, for which the name Coolia malayensis is proposed.},
	keywords = {benthic dinoflagellates, Coolia malayensis, ITS2 rRNA transcript, morphology, phylogeny},
	doi = {10.1111/j.1529-8817.2009.00778.x},
	author = {Chui-Pin Leaw and Po-Teen Lim and Kok-Wah Cheng and Boon-Koon Ng and Gires Usup}
}
@article {10,
	title = {Morphology and molecular phylogeny of Prorocentrum consutum sp. nov. (Dinophyceae), a new benthic dinoflagellate from South Brittany (Northwestern France)},
	journal = {Journal of Phycology},
	volume = {46},
	year = {2010},
	month = {02/2010},
	pages = {183-194},
	chapter = {183},
	abstract = {A new marine benthic Prorocentrum species from sandy habitats of South Brittany (northwestern France), P. consutum sp. nov., is described using LM and SEM and molecular phylogenetic analyses. Cells have a subcircular to broadly ovoid shape and are plainly flattened. They are 57-61 microns long and 52-55 microns wide. A central pyrenoid is present, and the kidney-shaped nucleus is positioned in the posterior region. In right valve view, the periflagellar area is deeply excavated, and the left valve forms a proinent apical ridge. The periflagellar area consists of nine platelets, and a small narrow collar is present around the flagellar pore. The ornamentation of this new species is very peculiar and is characterized by a ring of round areolae located at the peripherhy of the valves, each areola containing three or four pores. Apart from this ring of areolae, the cell surface is smooth and with scattered pores. Pores are not present in the center of the right or left valve. The intercalary band is generally narrow and faintly striated horizontally. The molecular phylogenetic position of P. consutum sp. nov. was inferred using SSU and LSU rDNA. In both analyses, this species branched with high support in the clade comprising species with a symmetric shape and appeared to be a sister group to that formed by P. lima and other tropical benthic species, such as P. arenarium, P. belizeanum, P. hoffmannianum and P. maculosum.},
	keywords = {dinoflagellates, LSU rDNA, molecular phylogeny, nuclear DNA, Prorocentrum, SEM, SSU rDNA, taxonomy},
	doi = {10.1111/j.1529-8817.2009.00774.x},
	author = {Nicolas Chomerat and Daniel Y. Sellos and Fr{\'e}d{\'e}ric Zentz and Elisabeth N{\'e}zan}
}
